Multiple axonal spike initiation zones in a motor neuron: serotonin activation

Abstract

The lateral gastric (LG) motor neuron of the stomatogastric nervous system of the crab Cancer borealis has a large soma in the stomatogastric ganglion (STG). The LG motor neuron makes inhibitory synaptic connections within the neuropil of the STG, and also projects to the periphery, where it innervates a series of muscles that control the movements of the lateral teeth of the gastric mill. The LG motor neuron has a spike initiation zone close to its neuropilar integrative regions, from which spikes propagate orthodromically to the muscles. Additionally, under certain conditions, the LG neuron can initiate spikes at peripheral axonal sites that can be 0.5-2.0 cm from the STG. Peripherally initiated spikes propagate antidromically into the STG and also propagate to the muscle. The peripheral spike initiation zones are often active in combined preparations in which the muscles are left attached. When the muscles are removed, depolarization of the LG soma together with 5-HT applied to the motor nerve also evokes peripheral spike initiation. At a given 5-HT concentration, the duration of the trains of antidromic spikes can be controlled by current injection into the soma, suggesting the presence of a slow voltage-dependent conductance in the LG axon. The antidromic spikes contribute to lengthening of the duration of contraction in some of the muscles innervated by the LG, but do not evoke IPSPs onto LG follower neurons. Thus, the LG neuron can send different signals to its peripheral and central targets.