Abstract
Multiple-pass cuvettes which provides a 6-12-fold increase in the sensitivity of spectrofluorimetric measurements are described. With these cuvettes, radiation losses by reflection and polarization are greatly decreased. The cuvettes can be placed directly into most commercial spectrofluorimeters. In the mirror cuvette, the exciting radiation is reflected by an aluminium or silver layer applied to the outer walls of the cuvette. In the cuvette based on total internal reflection, exciting radiation is reflected at the quartz/air boundaries of two attached prisms. Fluorescence spectra of rhodamine-B (1×10 −8 M) in ethanol illustrate the enhancement obtained and the improved accuracy of measurements of fluorescent lifetimes.
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