Abstract
In this paper we present a multimodal device for imaging fundus of human eye in vivo which combines functionality of autofluorescence by confocal SLO with Fourier domain OCT. Native fluorescence of human fundus was excited by modulated laser beam (λ = 473 nm, 20 MHz) and lock-in detection was applied resulting in improving sensitivity. The setup allows for acquisition of high resolution OCT and high contrast AF images using fluorescence excitation power of 50-65 μW without averaging consecutive images. Successful functioning of constructed device have been demonstrated for 8 healthy volunteers of different age ranging from 24 to 83 years old.
Highlights
Imaging of human eye in vivo by measuring fundus autofluorescence (FAF) is a diagnostic tool commonly used in ophthalmology
It provides complementary information to that gathered by other imaging techniques like fluorescein angiography and optical coherence tomography (OCT)
We observe the spatial distribution of intensity of fluorescence (Fig. 9(a)) similar to that reported in the literature [28] with the central minimum of fluorescence signal in the fovea caused by absorption of melanin and the macular pigment
Summary
Imaging of human eye in vivo by measuring fundus autofluorescence (FAF) is a diagnostic tool commonly used in ophthalmology. It provides complementary information to that gathered by other imaging techniques like fluorescein angiography and optical coherence tomography (OCT). Typical powers used for excitation of fundus autofluorescence in vivo are above 200 μW [3, 4] and images are obtained by averaging usually of 4-32 frames [5]. Optical Coherence Tomography OCT is a rapidly developing imaging technique [6]. It allows for cross-sectional imaging of weakly scattering samples with high sensitivity. Fourier domain OCT allows for high spatial resolution of imaging up to few microns in axial and lateral dimensions [7]. During past two decades Fourier domain OCT imaging of structure of the retina became one of the most widespread l imaging techniques in ophthalmology
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