Abstract
This work reports a multimodal system for label-free tissue diagnosis combining fluorescence lifetime imaging (FLIm), ultrasound backscatter microscopy (UBM), and photoacoustic imaging (PAI). This system provides complementary biochemical, structural and functional features allowing for enhanced in vivo detection of oral carcinoma. Results from a hamster oral carcinoma model (normal, precancer and carcinoma) are presented demonstrating the ability of FLIm to delineate biochemical composition at the tissue surface, UBM and related radiofrequency parameters to identify disruptions in the tissue microarchitecture and PAI to map optical absorption associated with specific tissue morphology and physiology.
Highlights
Cancer is the second leading cause of death in the United States with a total of 1,660,290 new cancer cases and 580,350 cancer deaths projected in 2013 [1]
This paper presents results from representative tissue conditions to demonstrate the ability of the multimodal system to evaluate pathological features of oral carcinoma
This study reports a multimodal system combining fluorescence lifetime imaging (FLIm), ultrasound backscatter microscopy (UBM) and photoacoustic imaging (PAI) techniques using a unique scanning probe device to integrate the optical and ultrasonic energy delivery-collection pathways
Summary
Cancer is the second leading cause of death in the United States with a total of 1,660,290 new cancer cases and 580,350 cancer deaths projected in 2013 [1]. Optical techniques have been employed to develop multimodal systems for in vivo imaging, including combination of time-resolved fluorescence spectroscopy and ultrasound [3], optical coherence tomography (OCT) and fluorescence imaging [4], fluorescence and MRI [5], and fluorescence intravital microscopy, dynamic light scattering and spectrally enhanced microscopy [6]. Optical imaging techniques provide biochemical and/or functional information with high sensitivity that can be complemented with depth dependent structural information from other modalities (such as ultrasound) which is otherwise not available due to limited penetration of light in tissue. Such multimodal techniques provide a means for rapid cancer diagnosis by providing holistic information about tissue pathology
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
