Multilayered structure based on poly(N,N-dimethyl-acrylamide-co-3,9-divinyl-2,4,8,10-tetraoxaspiro (5.5) undecane) prepared in a multiphase gelation process

Abstract

The study is devoted to the preparation of a multi-membrane gel system based on poly(N,N-dimethyl-acrylamide-co-3,9-divinyl-2,4,8,10-tetraoxaspiro (5.5) undecane) copolymer. The complex supra-structure was realized by layer by layer deposition technique, from the gel-core to the periphery by crosslinking poly(N,N-dimethyl-acrylamide-co-3,9-divinyl-2,4,8,10-tetraoxaspiro (5.5) undecane) (P(DMA-co-U)) with N,N'-methylene-bis-acrylamide and using as well β-cyclodextrin with double purpose as compound for constructing further the edge of the interfacial supramolecular assemblies and to improve the host-guest complexation of further bioactive compounds. Basic copolymer has dual thermo- and pH sensitive character. The multi-membrane 'onion-like' gel system was tested as matrix for embedding Norfloxacin. Hematologic, biochemical, immunologic studies used to determine the biocompatibility of multi-membrane gel structures loaded with drug that directly contact living tissue provided a reasonable assessment of safety as implants. Histopathologic exams did not reveal any disturbances of the normal liver architecture in animals with multi-membrane gel system containing Norfloxacin compared to control group with sterile cotton pellets implant. Good biocompatibility for the implants based on multi-membrane gel structures loaded with drug were achieved from the in vivo tests these suggesting that biomaterials may be suitable for in vivo use as a local drug delivery system.