Multigene Phylogeny and Morphology Reveal Three Novel Species and a Novel Record of Agaricus From Northern Thailand.

Chanyawat Jaichaliaw,Saisamorn Lumyong,Santhiti Vadthanarat,Jaturong Kumla,Nakarin Suwannarach

doi:10.3389/fmicb.2021.650513

Chanyawat Jaichaliaw, Saisamorn Lumyong + Show 3 more

Open Access

https://doi.org/10.3389/fmicb.2021.650513

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Abstract

Agaricus is a saprophytic mushroom genus widely distributed throughout the world. In this study, a survey of the Agaricus species carried out around Chiang Mai University in northern Thailand from 2018 to 2019 yielded 12 collections. Morphological characteristics and phylogenic analyses based on the internal transcribed spacers (ITS) and a fragment of the large subunit (LSU) of the nuclear ribosomal DNA (rDNA), and a fragment of the translation elongation factor 1-alpha (tef1) genes were investigated. The results revealed that these collections belong to six species including Agaricus erectosquamosus, Agaricus pallidobrunneus, Agaricus subrufescens, and three new species. Agaricus thailandensis sp. nov. was found to belong to Agaricus sect. Minores, which is placed in Agaricus subg. Minores. Aagricus pseudoerectosquamosus sp. nov. was placed in Agaricus sect. Brunneopicti within Agaricus subg. Pseudochitonia. Furthermore, Agaricus lannaensis remains an incertae sedis in Agaricus subg. Pseudochitonia. Additionally, this study was proposed that A. pallidobrunneus was discovered in Thailand for the first time. Full descriptions, color photographs, illustrations, and phylogenetic trees are provided.

Highlights

The genus Agaricus L. with Agaricus campestris L. as the type species was first proposed by Donk (1962)
Agaricus is widely distributed in both temperate and tropical areas throughout the world (Kerrigan et al, 2005, Kerrigan, 2016; Chen et al, 2015; Zhao et al, 2016; He et al, 2017, 2018a,b; Callac and Chen, 2018; Ling et al, 2021)
Morphological characteristics have been traditionally used in the identification of specimens of the Agaricus species

Summary

Introduction

The genus Agaricus L. with Agaricus campestris L. as the type species was first proposed by Donk (1962). This genus belongs to the family Agaricaceae of the order Agaricales. It is distributed worldwide and is commonly found in grasslands and forests (Kerrigan et al, 2005, Kerrigan, 2016; Parra, 2008, 2013; Zhao et al, 2012a, 2016; Chen et al, 2015; He et al, 2018a,b).

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