Multifunctional peptides derived from an egg yolk protein hydrolysate: isolation and characterization.

Aleksandra Zambrowicz,Bartosz Setner,Marta Pokora,Konrad Babij,Marek Szołtysik,Józefa Chrzanowska,Gert Lubec,Zbigniew Szewczuk,Tadeusz Trziszka,Anna Dąbrowska

doi:10.1007/s00726-014-1869-x

Abstract

An egg yolk protein by-product following ethanol extraction of phospholipids (YP) was hydrolyzed with pepsin to produce and identify novel peptides that revealed antioxidant, ACE inhibitory and antidiabetic (α-glucosidase and DPP-IV inhibitory) activities. The peptic hydrolysate of YP was fractionated by ion-exchange chromatography and reversed-phase high-pressure liquid chromatography. Isolated peptides were identified using mass spectrometry (MALDI-ToF) and the Mascot Search Results database. Four peptides of MW ranging from 1,210.62 to 1,677.88 Da corresponded to the fragments of Apolipoprotein B (YINQMPQKSRE; YINQMPQKSREA), Vitellogenin-2 (VTGRFAGHPAAQ) and Apovitellenin-1 (YIEAVNKVSPRAGQF). These peptides were chemically synthesized and showed antioxidant, ACE inhibitory or/and antidiabetic activities. Peptide YIEAVNKVSPRAGQF exerted the strongest ACE inhibitory activity, with IC50 = 9.4 µg/mL. The peptide YINQMPQKSRE showed the strongest DPPH free radical scavenging and DPP-IV inhibitory activities and its ACE inhibitory activity (IC50) reached 10.1 µg/mL. The peptide VTGRFAGHPAAQ revealed the highest α-glucosidase inhibitory activity (IC50 = 365.4 µg/mL). A novel nutraceutical effect for peptides from an egg yolk hydrolysate was shown.

Highlights

Introductiondiabetes mellitus (DM) is an endocrine system disease that causes metabolic disorders leading to multiple organ damage syndromes
Diet-related diseases such as cardiovascular disease, obesity, hypertension and diabetes mellitus (DM) represent significant health problems worldwide and there is evidence to suggest a possible common pathophysiology among type 2 DM, cardiovascular disease and oxidative stress.DM is an endocrine system disease that causes metabolic disorders leading to multiple organ damage syndromes
An effective strategy for the control of DM type 2 is the use of inhibitors of α-glucosidase which catalyzes the cleavage of d-glucose from oligosaccharides and disaccharides, and the inhibitors of dipeptidyl peptidase-4 (DPP-4) which participates in the rapid decomposition of GLP-1, a hormone increasing the secretion of insulin by the pancreatic beta cells and decreasing the secretion of glucagon after meal (Matsui et al 1996; Lacroix and Li-Chan 2012)

Summary

Introduction

DM is an endocrine system disease that causes metabolic disorders leading to multiple organ damage syndromes. An effective strategy for the control of DM type 2 is the use of inhibitors of α-glucosidase which catalyzes the cleavage of d-glucose from oligosaccharides and disaccharides, and the inhibitors of dipeptidyl peptidase-4 (DPP-4) which participates in the rapid decomposition of GLP-1, a hormone increasing the secretion of insulin by the pancreatic beta cells and decreasing the secretion of glucagon after meal (Matsui et al 1996; Lacroix and Li-Chan 2012). DM type 2 can lead to serious complications including organs damage, such as renal failure. Insulin resistance is related to hypertension (Matsui et al 1996)

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