Multifrequency Phase-Resolution For Total Luminescence Spectroscopy

Abstract

Multifrequency phase-resolved fluorescence spectroscopy is used to generate a multidimensional data array in which phase-resolved fluorescence intensity is measured as a function of emission wavelength and excitation wavelength at a series of excitation modulation frequencies. The dependence of the phase-resolved fluorescence intensity on fluorescence lifetime, modulation frequency and detector phase angle setting is examined. A model two-component system of benzo(k)fluoranthene and benzo(b)fluoranthene has been used to demonsrate the ability of phase-resolved fluorescence spectroscopy to selectively enhance the spectral contribution of a component as a function of fluorescence lifetime in total luminescence spectroscopyl, and is also discussed here.