Abstract
Although many phytochemicals have been used in traditional medicine, there is a great need to refresh the health benefits and adjust the shortcomings of herbal medicine. In this research, two herbal principles (Diosgenin and Glycyrrhiza glabra extract) coopted in the Nanostructured Lipid Carriers have been developed for improving the most desirable properties of herbal medicine—antioxidant and anti-inflammatory actions. The contribution of phytochemicals, vegetable oils and of lipid matrices has been highlighted by comparative study of size, stability, entrapment efficiency, morphological characteristics, and thermal behavior. According to the in vitro MTS and RTCA results, the dual herbal-NLCs were no cytotoxic toward endothelial cells at concentrations between 25 and 100 µg/mL. A rapid release of Glycyrrhiza glabra and a motivated delay of Diosgenin was detected by the in vitro release experiments. Dual herbal-NLCs showed an elevated ability to annihilate long-life cationic radicals (ABTS•+) and short-life oxygenated radicals (an inhibition of 63.4% ABTS•+, while the ability to capture radical oxygen species reached 96%). The production of pro-inflammatory cytokines was significantly inhibited by the newly herbals-NLC (up to 97.9% inhibition of TNF-α and 62.5% for IL-6). The study may open a new pharmacotherapy horizon; it provides a comprehensive basis for the use of herbal-NLC in the treatment of inflammatory diseases.
Highlights
Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu No 1, Virology Institute Stefan S
The preliminary characterization of lipid nanocarriers was represented by the comparative determination of the average diameters of Nanostructured lipid carriers (NLC) that present a single incorporated plant principle, referred as NLC-individual (NLC-I/II-DSG and NLC-I/II-GlyG) and those that co-encapsulate both plant principles, hydrophilic and lipophilic, referred as NLCdual (NLC-I/II-DSG-GlyG)
A comparative analysis of Zave characteristic of the two categories of NLC, shows insignificant differences between them, both vegetable oils being suitable for obtaining lipid nanocarriers that have desired encapsulation characteristics for entrapping both mixtures of bioactive phytochemicals principles, DSG and GlyG
Summary
Faculty of Applied Chemistry and Materials Science, University Politehnica of Bucharest, Polizu No 1, Virology Institute Stefan S. Two herbal principles (Diosgenin and Glycyrrhiza glabra extract) coopted in the Nanostructured Lipid. According to the in vitro MTS and RTCA results, the dual herbal-NLCs were no cytotoxic toward endothelial cells at concentrations between 25 and. The production of pro-inflammatory cytokines was significantly inhibited by the newly herbals-NLC The study may open a new pharmacotherapy horizon; it provides a comprehensive basis for the use of herbal-NLC in the treatment of inflammatory diseases
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