Abstract
The major-to-trace elements in the human blood serum samples, collected from the healthy volunteers as well as from the patients of specific diseases, were determined by ICP-AES (inductively coupled plasma atomic emission spectrometry) and ICP-MS (inductively coupled plasma mass spectrometry) after acid digestion. The whole blood samples were collected with the silicone-coated syringes and centrifuged at 3000 rpm to obtain the serum samples. Then, the blood serum samples were digested with nitric acid on an aluminum bath. Finally, the residue of blood serum was dissolved with 0.1 M nitric acid to subject to the analyses by ICP-AES and ICP-MS. The analytical results were summarized as the radar charts, which indicated the relative concentrations of 14 elements in order to perform the multielement correlation analyses for medical diagnosis. Such multielement radar charts for specific disease patients were compared to those for the healthy volunteers. It was found that the concentrations of P and Cu in sera collected from healthy females were higher than those from healthy males, while those of Fe, Zn, Mo and Ag in sera from the former were lower than those from the latter. Furthermore, the significant increases in Cu, Se and Mo were clearly observed for non-Hodgkin’s lymphoma (blood cancer) patients.
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