Multidrug-Resistant Proteus mirabilis Strain with Cointegrate Plasmid.

Andrey Shelenkov,Lyudmila Petrova,Yulia Mikhaylova,Mikhail Zamyatin,Valeria Fomina,Vasiliy Akimkin

doi:10.3390/microorganisms8111775

Andrey Shelenkov, Lyudmila Petrova + Show 4 more

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https://doi.org/10.3390/microorganisms8111775

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Journal: Microorganisms	Publication Date: Nov 12, 2020
Citations: 14	License type: CC BY 4.0

Affiliation: Central Research Institute of Epidemiology

Abstract

Proteus mirabilis is a component of the normal intestinal microflora of humans and animals, but can cause urinary tract infections and even sepsis in hospital settings. In recent years, the number of multidrug-resistant P. mirabilis isolates, including the ones producing extended-spectrum β-lactamases (ESBLs), is increasing worldwide. However, the number of investigations dedicated to this species, especially, whole-genome sequencing, is much lower in comparison to the members of the ESKAPE pathogens group. This study presents a detailed analysis of clinical multidrug-resistant ESBL-producing P. mirabilis isolate using short- and long-read whole-genome sequencing, which allowed us to reveal possible horizontal gene transfer between Klebsiella pneumoniae and P. mirabilis plasmids and to locate the CRISPR-Cas system in the genome together with its probable phage targets, as well as multiple virulence genes. We believe that the data presented will contribute to the understanding of antibiotic resistance acquisition and virulence mechanisms for this important pathogen.

Highlights

Proteus mirabilis is a member of the Morganellaceae family of gram-negative bacilli
This study presents a detailed analysis of clinical multidrug-resistant ESBL-producing P. mirabilis isolate using short- and long-read whole-genome sequencing, which allowed us to reveal possible horizontal gene transfer between Klebsiella pneumoniae and P. mirabilis plasmids and to locate the CRISPR-Cas system in the genome together with its probable phage targets, as well as multiple virulence genes
In contrast to ESKAPE pathogens, P. mirabilis is not considered as a reservoir of plasmid-encoded antimicrobial resistance (AMR) genes by some authors (e.g., Reference [42]), but recent reports have demonstrated the possibility of frequent plasmid-mediated AMR gene transfer for this species [43,44]

Summary

Introduction

Proteus mirabilis is a member of the Morganellaceae family of gram-negative bacilli. Before 2016, it had been assigned to Enterobacteriaceae family. The ability of Proteus organisms to produce urease and to alkalinize the urine by hydrolyzing urea to ammonia makes them effective in producing an environment in which they can survive. This leads to precipitation of organic and inorganic compounds, which leads to struvite stone formation. Among various Proteus species, P. mirabilis isolates are clinically significant and usually responsible for urinary tract and wound infections. They are the fifth most common cause of nosocomial urinary tract infections and sepsis in hospitalized individuals [3]

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