Multidrug resistance found in extended-spectrum beta-lactamase-producing Enterobacteriaceae from rural water reservoirs in Guantao, China.

Hongna Zhang,Shuyuan Guo,Yufa Zhou,Weishan Chang

doi:10.3389/fmicb.2015.00267

Abstract

Extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae have been isolated from humans and animals across the world. However, data on prevalence of ESBL-producing Enterobacteriaceae from rural water reservoirs is limited. This study aimed to isolate and characterize ESBL-producing Enterobacteriaceae in rural water reservoirs in Guantao, China. ESBL-producing Enterobacteriaceae were found in 5 (16.7%) of 30 sampled rural water reservoirs. Sixty-six individual isolates expressing an ESBL phenotype were obtained in the present study. Species identification showed that 42 representatives of Escherichia coli, 17 Klebsiella pneumoniae, 4 Raoultella planticola, and 3 Enterobacter cloacae. Twenty isolates contained a single bla gene, including CTX-M (17 strains), TEM (2 strains), and SHV (1 strain). Forty-six isolates contained more than one type of beta-lactamase genes. ESBL-producing Enterobacteriaceae isolated in this study were all multidrug resistant. These findings indicated that the serious contamination of ESBL-producing Enterobacteriaceae in rural water reservoirs existed in Guantao, China.

Highlights

The rational use of antibiotics helps control infectious diseases of humans and animals
Detection of ESBL-producing Enterobacteriaceae Extended-spectrum beta-lactamase-producing Enterobacteriaceae were detected in five water reservoirs of two rural communities (D: 2, E: 3)
No ESBL-producing Enterobacteriaceae were found in the other reservoirs, which were far away from rural villages and animal farms

Summary

Introduction

The rational use of antibiotics helps control infectious diseases of humans and animals. Extended-spectrum beta-lactamases (ESBLs), resulting from amino acid substitutions in TEM-1, TEM-2, and SHV-1 enzymes were described in the 1980s and 1990s (Bush and Jacoby, 2010). Extended-spectrum beta-lactamase-producing Enterobacteriaceae can spread between humans via contaminated food or water (Oteo et al, 2010; Piednoir et al, 2011) and acquire resistance to antibiotics by plasmids, transposons or other mobile vectors that carry resistance elements (Oteo et al, 2010; Peirano et al, 2012). Once ESBL-producing Enterobacteriaceae enter the intestine of humans and animals via drinking water, these bacteria could lead to the spread of resistance genes and to serious infections

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