Year-end Sale % OFF 
Abstract
Transference of antimicrobial resistance gene determinants by integrons is one of the important factors that can contribute to the increase in multidrug-resistant bacteria. The present study aimed to investigate 56 strains of animal-derived E. coli strains obtained from fecal materials of slaughtered food animals for their resistance to commonly used antimicrobial agents for presence of integrons and detection of mercury reductase gene (MerA) as indicative for presence of transposons. Multidrug resistance (MDR) was found in 38 (68%) of the strains as indicated by disk diffusion method. The most frequent resistance patterns was to Ampicillin, Sulfonamides, Nalidixic acid, Cephalosporin's, Tetracycline, and Aamoxicillin/Clavulanic acid. None of tested strains were resistant to Cefepime or Imipenem. The polymerase chain reaction (PCR) results showed the presence of integrons in 26 (68.4%) of MDR strains tested as well as the mercury resistance gene in 12 (46.2%) of strains with integrons, indicating the role of Tn21 transposon in dissemination of the integrons and their gene cassettes within the samples studied. Our results showed high resistance rate of E. coli from food animals to routine antibiotics and indicate that integrons and transposon are common among MDR isolates of food producing isolates in Saudi Arabia.
Highlights
Antibiotic resistance is a threat for therapy failure in human medicine
All the 56 E. coli isolates tested for susceptibility to antibiotics and screened for the presence of integrons and transposon
Antibiotic susceptibility assay The antibiotic resistance behavior of the isolated strains was determined on cation-adjusted MuellerHinton agar (Hi-Media) using disk diffusion method according to the standards and interpretive criteria described by Clinical and Laboratory Standards Institute (CLSI, 2013)
Summary
Antibiotic resistance is a threat for therapy failure in human medicine. The use of antimicrobial substances in animal husbandry is considered the most important factor for debate due to its possible implications on emergence, selection and dissemination of resistant bacteria from food animals to human (Threlfall et al, 1993; Johnston, 1998).One of the most recent adopted actions worldwide is to control and analyze the antibiotic resistance in individual countries, it is necessary to have adequate control systems which would integrate the data concerning antibiotic resistance of bacteria isolated from animals. The majority of resistance genes encoding a wide variety of resistance mechanisms are carried by mobile genetic elements such as integrons, transposons, plasmids (Iyer et al, 2013) which favors the transfer of MDR genotypes between commensals and pathogens, animals and humans. Integrons are genetic structures capable of integrating or mobilizing gene cassettes encoding antibiotic resistance determinants (Carattoli, 2001). Integrons are a major mechanism for the spread of multidrug resistance from both clinical isolates and normal flora of food animals, as well as in human clinical specimens (Goldstein et al, 2001). Each with different int genes have been identified (intI1, intI2, and intI3) that are known to be associated with antibiotic resistance (Mazel, 2006)
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
