Abstract
Modern streak camera based pulse fluorometers readily allow data collection in two-dimensions. We have developed a modular instrument that is easily adapted for recording time-resolved emission spectra, fluorescence depolarization data, and time series of time-resolved fluorescence decays. The instrument is based on a synchroscan streak camera and a passively mode-locked Ti:Sapphire laser using second harmonic generation and third harmonic generation to extend the excitation wavelength range to the visible and ultraviolet. The performance of the instrument was tested using standard biological samples. The dynamic range is typically 600:1, and the detection limit is less than 1 fW. With respect to the fluorescence decay kinetic (first time axis), the temporal resolution is better than 10 ps, and rotational correlation times of 50 ps are measured routinely. For the second time axis, the maximum time resolution is approximately 2 ms.
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