Multi-component quantitative analysis of fluorescent mixtures not obeying Beer's law

Abstract

The determination of mixtures by a linear least-squares regression analysis is a well established technique. However, this mathematical approach depends on the individual components obeying the Beer-Lambert law. Although this assumption is generally true in UV absorption, the analysis of mixtures by fluorescence spectrometry presents a number of problems. Distortion of the sample spectra can occur through a variety of reasons, including energy transfer. By using standards that are mixtures of the components, rather than pure components, compensation can be made for non-linear deviations.