Abstract

BackgroundThe multicomponent meningococcal serogroup B vaccine (4CMenB) is currently indicated for active immunization against invasive meningococcal disease caused by Neisseria meningitidis serogroup B (MenB). However, genes encoding the 4CMenB antigens are also variably present and expressed in strains belonging to other meningococcal serogroups. In this study, we evaluated the ability of antibodies raised by 4CMenB immunisation to induce complement-mediated bactericidal killing of non-MenB strains. MethodsA total of 227 invasive non-MenB disease isolates were collected between 1 July 2007 and 30 June 2008 from England and Wales, France, and Germany; 41 isolates were collected during 2012 from Brazil. The isolates were subjected to genotypic analyses. A subset of 147 isolates (MenC, MenW and MenY) representative of the meningococcal genetic diversity of the total sample were tested in the human complement serum bactericidal antibody assay (hSBA) using sera from infants immunised with 4CMenB. ResultsSerogroup and clonal complex repertoires of non-MenB isolates were different for each country. For the European panel, MenC, MenW and MenY isolates belonged mainly to ST-11, ST-22 and ST-23 complexes, respectively. For the Brazilian panel, most MenC and MenW isolates belonged to the ST-103 and ST-11 complexes, respectively, and most MenY isolates were not assigned to clonal complexes. Of the 147 non-MenB isolates, 109 were killed in hSBA, resulting in an overall coverage of 74%. ConclusionThis is the first study in which 147 non-MenB serogroup isolates have been analysed in hSBA to evaluate the potential of a MenB vaccine to cover strains belonging to other serogroups. These data demonstrate that antibodies raised by 4CMenB are able to induce bactericidal killing of 109 non-MenB isolates, representative of non-MenB genetic and geographic diversity. These findings support previous evidence that 4CMenB immunisation can provide cross-protection against non-MenB strains in infants, which represents an added benefit of 4CMenB vaccination.

Highlights

  • Invasive meningococcal disease (IMD) remains a major public health concern worldwide

  • We evaluated the ability of antibodies raised by 4CMenB immunisation to induce complement-mediated bactericidal killing of non-meningitidis serogroup B (MenB) strains

  • Quadrivalent MenACWY glycoconjugate vaccines have been approved for use for several years, and tailormade vaccines composed of meningococcal outer membrane vesicles (OMV) have proven largely efficacious against MenB clonal outbreaks in Brazil, Norway, Cuba, New Zealand and France [3,4,5,6,7]

Read more

Summary

Introduction

Invasive meningococcal disease (IMD) remains a major public health concern worldwide. The introduction of an affordable and highly efficacious MenA glycoconjugate vaccine (MenAfriVac, Serum Institute of India) has drastically reduced the burden of MenA-IMD in the so-called sub-Saharan African meningitis belt [8,9] Despite these impressive strides, meningococcal vaccination uptake in most developing countries has been fairly slow and a sampling of global IMD epidemiology suggests that all six serogroups still account for significant IMD-related morbidity and mortality. Conclusion: This is the first study in which 147 non-MenB serogroup isolates have been analysed in hSBA to evaluate the potential of a MenB vaccine to cover strains belonging to other serogroups These data demonstrate that antibodies raised by 4CMenB are able to induce bactericidal killing of 109 non-MenB isolates, representative of non-MenB genetic and geographic diversity.

Methods
Results
Discussion
Conclusion
Full Text
Published version (Free)

Talk to us

Join us for a 30 min session where you can share your feedback and ask us any queries you have

Schedule a call