Abstract
We have developed multicolour hybrid DNA probes employing green, yellow and orangecolour quantum dot conjugated molecular beacons with black hole quencher 2. Optical andelectrophoretic characterization revealed fluorescent energy transfer that follows the FRETmechanism with single nucleotide discrimination. Target DNA identification was observedto be highly sensitive up to 8 ng in gel electrophoresis. Comparison with the conventionalorganic dye SYBR Gold™ showed that our hybrid nanoprobes exhibit more stableperformance with less background signal.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.