Abstract
A novel multichannel surface plasmon resonance (SPR) biosensor with spectral interrogation and wavelength division multiplexing of sensing channels is presented. A special design of SPR prism element is used to enable wavelength division multiplexing of serial sensing channels. In addition to increasing the number of sensing channels, this approach allows discrimination of the sensor response due to analyte–receptor binding from interfering background refractive index fluctuations. A novel eight-channel SPR sensor combining the wavelength division multiplexing of serial sensing channels with the conventional parallel channel architecture is described. Application of this multichannel SPR sensor for simultaneous detection of multiple analytes is described.
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