Abstract
An on-chip spectral surface plasmon resonance (SPR) biosensor with three parallel channels has been built for biomolecule detections. It is capable of updating intensity baselines to suppress errors induced by light source instability. In order to minimize structure and reduce positional regression uncertainty, the scan operations are achieved by timely changing optical media instead of conventional mechanical scan. The detection results of human immunoglobulin G (hIgG) demonstrated that the baseline updating improves the limitation of detection of hIgG concentration from ~86.1 to ~17.4 ng/mL.
Highlights
IntroductionAnalytical devices have shown minimization and integration trends. Different from routine analytical methods represented by tube and microtiter plate, the micrometer-scale total analysis systems (μTAS) or so called lab-chip devices, which integrate novel sensing units and offer significant advantages, such as miniaturization, saving of analyte samples, low power consumption, on-site testing, and rapidity of assay, have attracted more and more interest.[1]
Nowadays, analytical devices have shown minimization and integration trends
Different from routine analytical methods represented by tube and microtiter plate, the micrometer-scale total analysis systems or so called lab-chip devices, which integrate novel sensing units and offer significant advantages, such as miniaturization, saving of analyte samples, low power consumption, on-site testing, and rapidity of assay, have attracted more and more interest.[1]
Summary
Analytical devices have shown minimization and integration trends. Different from routine analytical methods represented by tube and microtiter plate, the micrometer-scale total analysis systems (μTAS) or so called lab-chip devices, which integrate novel sensing units and offer significant advantages, such as miniaturization, saving of analyte samples, low power consumption, on-site testing, and rapidity of assay, have attracted more and more interest.[1]. Spectral SPR sensors have become widely utilized analytical tools in biomedical research due to high performance and easy applications by using commercial spectrometer instruments.[2] For multianalyte detection and parallel reference assay capabilities, sensor systems are usually designed as multichannel structures. Multichannel scan mechanisms, which employ wavelength-division multiplexing,[3] multiple fiber receptors,[4] and mechanical movement of optics,[5] have been reported. These methods lead to complicated structures, more components, larger device sizes, and even induce positional regression uncertainty errors, and impair the convenience in on-site biomedical measurements.[6] In order to alleviate these drawbacks, we designed a simple and compact multichannel SPR biochip. The device performances in biomedical sensing were demonstrated by a representative detection of
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