Multichannel cell thermometry system based on transparent platinum resistance for real-time visual monitoring energy metabolism in vascular endothelial cells

Abstract

Temperature variation is a crucial indicator of cellular physiological processes, however obtaining reliable and stable cellular temperature information over extended period is challenging. Additionally, existing techniques for cell temperature measurement often have limited functionality, making it difficult to detect and analyze cell temperature and other physicochemical properties simultaneously. In this study, we have developed a cell thermometry system based on a low-noise and transparent substrate temperature sensor, which maintains a long-term stable working status. Moreover, it can be integrated with an imaging device to capture optical microscopic information at the same time. Using this system, we observed a strong correlation between changes in mitochondrial membrane potential and cell temperature under the influence of carbonyl cyanide m-chlorophenylhydrazone (CCCP). This novel cell temperature measurement system, capable of acquiring optical information, can provide valuable reference data for studying cell temperature-related physiological processes and screening drugs.