Abstract
Multicellular tumour spheroids (MTS) from 4 mouse tumours (Line 1 lung carcinoma; a fibrosarcoma, FSA; a mammary carcinoma, MCa-11; and SV40-transformed fibroblasts, SV-A31) WEre injected into the abdominal cavity of normal, immunized or tumour-bearing syngeneic mice, recovered after 4-48 h, and their growth measured in vitro for 7-16 days. Both normal and immunized mice inhibited MTS growth, but there was no correlation between the two types of inhibition, suggesting that different immunological processes were involved. For example, the greatest inhibition by normal mice was seen for the weakly immunogenic MCa-11, and the highly immunogenic tumour, SV-A31, was only moderately inhibited. However, the summed inhibition of MTS growth in normal and sensitized hosts corresponded to the behaviour of tumours as s.c. transplants; i.e., was inversely related to the malignancy of the same tumours. The inhibition of MTS by mice bearing identical early tumours (FSA or MCa-11) was comparable to that in immunized mice. Histological sections of SV-A31 MTS in normal or immunized hosts revealed the infiltration of MTS by various types of host cells, mostly polymorphonuclears, macrophages and lymphocytes.
Highlights
Summary.-Multicellular tumour spheroids (MTS) from 4 mouse tumours (Line 1 lung carcinoma; a fibrosarcoma, FSA; a mammary carcinoma, MCa-11; and SV40-transformed fibroblasts, SV-A31) were injected into the abdominal cavity of normal, immunized or tumour-bearing syngeneic mice, recovered after 4-48 h, and their growth measured in vitro for 7-16 days
For FSA and MCa- 11 tumours growth curves are included of MTS exposed to mice bearing corresponding tumours
MTS, as any in vitro growing tumour tissue, lack some characteristics of tumours in vivo, they do have many properties (morphological, metabolic and kinetic -which closelv resemble those of solid tumours (Sutherland & I)urand, 1976; Yuhas et al, 1977; Yuhas & Li, 1978)
Summary
Implicates the question of how the local, Animals.-BALB/cJ and C3H/HeJ female microenvironmental factor may influence mice were obtained from the Jackson Laboraimmunological reaction, which is largely tory (Bar Harbor, Maine). Mice immunized to FSA or MCa- 11 were given 3 weekly injections of 1-2 x 107 X-irradiated (10,000 rad) tumour cells. Mice which rejected this dose of Line 1 cells (about 80%) within 25-30 days of challenge were considered immune and used in experiments. A certain percentage of Line 1, SV-A31 and FSA MTS (mainly those recovered from immunized mice) completely degenerated during the observation period. Those MTS were not included in growth curves, but their incidence is shown separately. The number of lymphoid cells recovered from MTS was probably too low to produce any significant inhibition of colony growth after the plating Identification was checked in thin sections by transmission electron microscopy
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