Abstract
An optical-resolution photoacoustic microscope with modulated CW laser diodes allowing multi-channel imaging is presented that can be used for both imaging biological tissues and for targeted photo-dynamic therapy (PDT) varying the optical power and exposure time. The effects of this therapy are immediately monitored in order to optimize the time of irradiation. After the description of the experimental setup, in vitro and in vivo applications are presented on a synthetic sample and on the mouse ear using hemoglobin as endogenous and methylene blue as exogenous dye for imaging and PDT, respectively.
Highlights
In clinic, there is an important need for handheld imaging equipment for the detection of microscopic effects of anti-cancer therapies
PA-imaging has the potential to be low cost, and handheld for in vivo imaging with either a microscopic optical resolution or with a mesoscopic acoustic resolution. In this preclinical proof of principle study, we focus on optical-resolution photoacoustic microscopy (OR-PAM) using modulated CW laser diodes
We designed a photoacoustic microscope combining the beams of two laser diodes
Summary
There is an important need for handheld imaging equipment for the detection of microscopic effects of anti-cancer therapies. These have multiple effects on tissue parameters, concerning tumor (micro)-vessels, different cell types, and inflammation processes. These effects are either anti- or pro-tumoral and often stimulate recurrence in time [1]. Recent clinical studies have shown that microscopic details of the tumor vessels in human breast cancer can be screened by photo-acoustic (PA) imaging to a depth of a few centimeters [2]. PA-imaging has the potential to be low cost, and handheld for in vivo imaging with either a microscopic optical resolution or with a mesoscopic acoustic resolution
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