Multi-wavelength interference phase imaging for automatic breast cancer detection and delineation using diffuse reflection imaging

Abstract

Millions of women globally are impacted by the major health problem of breast cancer (BC). Early detection of BC is critical for successful treatment and improved survival rates. In this study, we provide a progressive approach for BC detection using multi-wavelength interference (MWI) phase imaging based on diffuse reflection hyperspectral (HS) imaging. The proposed findings are based on the measurement of the interference pattern between the blue (446.6 nm) and red (632 nm) wavelengths. We consider implementing a comprehensive image processing and categorization method based on the use of Fast Fourier (FF) transform analysis pertaining to a change in the refractive index between tumor and normal tissue. We observed that cancer growth affects tissue organization dramatically, as seen by persistently increased refractive index variance in tumors compared normal areas. Both malignant and normal tissue had different depth data collected from it that was analyzed. To enhance the categorization of ex-vivo BC tissue, we developed and validated a training classifier algorithm specifically designed for categorizing HS cube data. Following the application of signal normalization with the FF transform algorithm, our methodology achieved a high level of performance with a specificity (Spec) of 94% and a sensitivity (Sen) of 90.9% for the 632 nm acquired image categorization, based on preliminary findings from breast specimens under investigation. Notably, we successfully leveraged unstained tissue samples to create 3D phase-resolved images that effectively highlight the distinctions in diffuse reflectance features between cancerous and healthy tissue. Preliminary data revealed that our imaging method might be able to assist specialists in safely excising malignant areas and assessing the tumor bed following resection automatically at different depths. This preliminary investigation might result in an effective "in-vivo" disease description utilizing optical technology using a typical RGB camera with wavelength-specific operation with our quantitative phase MWI imaging methodology.