Multi-view 3D reconstruction of seedling using 2D image contour

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3D reconstruction of seedling can provide comprehensive and quantitative spatial structure information, offering an effective digital tool for breeding research. However, accurate and efficient reconstruction of seedling is still a challenging work due to limited performance of depth sensor for seedling with small-size stem and unavoidable error for multi-view point cloud registration. Therefore, in this paper, we propose an accurate multi-view 3D reconstruction method for seedling using 2D image contour to constrain 3D point cloud. The rotation axis is calibrated and optimised by minimising point-to-contour distance between 2D image contour and projected exterior points from 3D point cloud. Then, to remove outliers and noise, we introduce the seedling mask of 2D image to constrained and delete projected outlier points of 3D model from corresponding view. Furthermore, we propose a residual-guided method to recognise missing region for 3D model and complete 3D model of small-size stem. Finally, we can obtain an accurate 3D model of seedling. The reconstruction accuracy is evaluated by average distance between projected contour of 3D model and 2D image contour of all views (0.3185 mm). Then, the phenotypic parameters were calculated from 3D model and the results are close to manual measurements (Plant height: R2 = 0.98, RMSE = 2.3 mm, rRMSE = 1.52%; Petioles inclination angle: R2 = 0.99, RMSE = 0.73°, rRMSE = 1.41%; Leaf area: R2 = 0.66, RMSE = 1.05 cm2, rRMSE = 7.63%; Leaf inclination angle: R2 = 0.99, RMSE = 1.01°, rRMSE = 1.72%; Stem diameter: R2 = 0.95, RMSE = 0.12 mm, rRMSE = 5.43%). Breeders can improve the selection of more resilient varieties and cultivars to different growing conditions starting from the dynamic analysis of their phenotype.