Multi-tissue Siberian sturgeon RNA sequencing data.

Christophe Klopp,Denise Vizziano-Cantonnet,Cédric Cabau,André Lasalle,Gonzalo Greif,Santiago Di Landro

doi:10.1016/j.dib.2020.105820

Abstract

Siberian sturgeon, Acipenser baerii, is a commercially valuable fish for flesh and caviar production and a threatened species. We produced transcriptomic data for ten tissues with relevance to puberty, reproduction, early development, growth and food intake. The data includes RNA-Seq read sets of brain, pituitary, anterior-kidney, kidney, stomach, liver, heart, embryonic, pre-larval, and immature gonad sequences. Tissues were collected from sex differentiated fish (17 to 42 months of age, 66 to 85 cm) RNA was extracted and sequenced. Our purpose is to facilitate fundamental studies of sturgeon physiology to wild and aquaculture populations management.

Highlights

Christophe Klopp a, Cédric Cabau c, André Lasalle b, Santiago Di Landro b, Gonzalo Greif c, Denise Vizziano-Cantonnet b,∗
Biology Transcriptomics, physiology and aquaculture Raw RNA-Seq data and assembled reference transcriptome assembly Illumina MiSeq Illumina HiSeq 2500 Raw data and assembly information Tissues collected from immature fish: pituitary, anterior-kidney, kidney, stomach, liver, gonads Tissues collected from gametogenetic fish: brain Tissues collected from sex undifferentiated fish: embryonic, pre-larval Tissues sequenced in a pool: pituitary, testis, ovary, anterior kidney, kidney, heart, stomach, liver
97.16 (1) Number of reads (2) Alignment rate: is the number of sequences aligned on the de novo transcriptome reference divided by the total number of sequences of the sample expressed in percent (3) Q20 ratio is the number of raw read base pairs having a quality score equal or over 20 divided by the total number of read base pairs of the sample expressed in percent

Summary

Data accessibility

Biology Transcriptomics, physiology and aquaculture Raw RNA-Seq data and assembled reference transcriptome assembly Illumina MiSeq Illumina HiSeq 2500 Raw data and assembly information Tissues collected from immature fish: pituitary, anterior-kidney, kidney, stomach, liver, gonads Tissues collected from gametogenetic fish: brain Tissues collected from sex undifferentiated fish: embryonic, pre-larval Tissues sequenced in a pool: pituitary, testis, ovary, anterior kidney, kidney, heart, stomach, liver. The dataset will facilitate research on topics of interest in Siberian sturgeon aquaculture such as puberty, reproduction, growth, food intake, and immunology Insights into these processes will improve management of both wild and aquaculture populations. We present a novel Siberian sturgeon multi-tissue data set including brain, pituitary, gonadal, liver, stomach, kidney, anterior kidney, heart, embryonic, and pre-larval transcriptomes, with the goal of facilitating crucial research on topics of interest in sturgeon physiology, such as puberty, reproduction, growth, food intake, and in immunology. (1) Number of reads (2) Alignment rate: is the number of sequences aligned on the de novo transcriptome reference divided by the total number of sequences of the sample expressed in percent (3) Q20 ratio is the number of raw read base pairs having a quality score equal or over 20 divided by the total number of read base pairs of the sample expressed in percent

Ethics statement

Experimental animals and rearing procedures

Findings

Transcriptome assembly