Abstract
HighlightsA framework nucleic acid delivery system was developed through self-assembly, which can deliver antisense oligonucleotides against multiple targets in bacterial cells.The ASOs-tFNAs (750 nM) was found to simultaneously inhibit the expression of gtfBCD, gbpB, and ftf, and significantly reduce the extracellular polysaccharide synthesis and biofilm thickness.
Highlights
Biofilms represent structured bacterial communities attached to an extracellular matrix secreted by the bacteria on the surface of a living or inanimate body [1]
The successful self-assembly of ASOstFNA was verified by non-denaturing polyacrylamide gel electrophoresis (Fig. 1a) and confirmed by the slower migration of antisense oligonucleotides (ASOs)-tetrahedral FNA (tFNA) compared to ssDNA or a combination of two, three, or four strands
The morphology of tFNA and ASOs-tFNA was characterized by atomic force microscopy (AFM) (Fig. 1e), and an approximate size of 10 nm was calculated for the ASOs-tFNA monomer
Summary
Biofilms represent structured bacterial communities attached to an extracellular matrix secreted by the bacteria on the surface of a living or inanimate body [1]. Various such drugs, including aptamers or cytosine-phosphate-guanine, have been designed for FNAs to provide anticancer or immunostimulatory activities [21,22,23] Based on these factors, we have developed a new drug delivery system that targets biofilms. Secretion of EPS in the early stages of S. mutans biofilm formation is regulated mainly by the glucosyltransferase gene family (gtfB, gtfC, gtfD) and the glucan-binding protein gbpB gene [27]. It confirms the vast potential of the system for the treatment of bacterial infections
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