Abstract

The interaction of three different fluorescent molecules viz, 2,5-dimethoxybenzaldehyde (DMB), fluorescein (Flu) and rhodamine B (RhB) with the surfactant systems of varied nature has been explored using steady state spectrofluorometry. Cosolubilization studies have been carried out to understand the micellar effects on the spectral properties of flourophores in their mixed state within the micelles and accordingly evaluated for their energy transfer processes. FRET studies have been carried out between DMB-Flu and Flu-RhB and thus two new FRET pairs have been established using micellar systems as scaffolds. It has been found that the nature of surfactant has a significant effect on the FRET efficiency of both the pairs. Herein, we also demonstrate the three step FRET taking place simultaneously from DMB to RhB via an intermediate Flu which otherwise does not occur due to the lack of spectral overlap between DMB and RhB and it is operative in all the micellar systems studied. This three component FRET so obtained can have vast applications in biological systems.

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