Abstract
.A multi-spectral laser speckle contrast imaging (MS-LSCI) system is proposed using only a single wavelength-swept laser, which provides both highly coherent and multi-spectral outputs to simultaneously generate laser speckle contrast images and multi-spectral images, respectively. Using a laser light swept from 770 to 821 nm at a repetition rate of 5 Hz and a CCD camera of 335 fps, 67 multi-spectral frame images are acquired in 0.76 nm wavebands over 51 nm spectral range. The spectral sub-windowing method of single wavelength-swept laser source is used to solve the lack of spectral information from a few individual light sources, which is a limitation of conventional MS-LSCI systems. In addition to the speckle flow index from the LSCI frames, the multi-spectrally encoded images can generate additional images of spectral absorbance. To further examine the performance of the MS-LSCI system, an in vivo cuff-induced ischemia experiment was conducted to show the real-time imaging of hemodynamic and blood oxygen saturation changes simultaneously over the entire field of view.
Highlights
Laser speckle contrast imaging (LSCI) is a simple and useful imaging technique that utilizes the speckle pattern randomly produced on the image sensor from a highly coherent light source.[1]
Since the standard deviation of the speckle contrast influences the sensitivity of flow velocity, it can be difficult to measure the change in flow velocity when the deviation of the speckle contrast is greater than that of the flow
Under the same phantom conditions, the camera exposure time is set to 5 ms by adjusting the camera frame-rate to 200 fps for maximum signal to noise ratio (SNR), and the region of interest (ROI) of the acrylic tube is set to 8 × 8 pixels for flow velocity detection, which corresponds to an area of 0.5 × 0.5 mm[2] on the sample
Summary
Laser speckle contrast imaging (LSCI) is a simple and useful imaging technique that utilizes the speckle pattern randomly produced on the image sensor from a highly coherent light source.[1]. This technique collects a series of images of the target over multiple wavelengths from an image sensor, such as a charge-coupled device (CCD) or a complementary metal-oxide semiconductor (CMOS) sensor, to analyze the composition of the target by acquiring spectrally distributed information, such as the reflectance and absorbance.[4,5] Recently, there have been reports of a few multi-modal imaging modalities combining LSCI and MSI systems.[6,7,8]
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