Abstract
General rate equations have been derived, which describe transport of a substrate via a n-site carrier in the presence of other compounds having affinity to the carrier. The n-site carrier can occur in two different states allowing coupling with metabolism. When the rate of transformation of the two states is high relative to the rates of carrier transfer across the cell membrane or when the rates of formation of one of the two states at the medium side of the cell membrane is very high, then the transport process can be described formally by the enzyme kinetics for an irreversible enzyme process catalyzed by an enzyme occurring in only one state. The meaning of the “kinetic constants”, however, has been changed. These “constants” are complex functions of rate constants, equilibrium constants, the metabolic state of the cells and the intracellular concentrations of the substrate and of other compounds having also affinity to the two carriers.
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