Multi potent aromatic nano colloid: synthesis, characterization and applications

Salman Al Farzi Mohamed Sheik Meeran,Hemalatha Srinivasan,Ruckmani Kandasamy,Shruthy Priya Prakash,Ranjani Soundhararajan,Waseem Mohammad

doi:10.1186/s13568-020-01104-5

Salman Al Farzi Mohamed Sheik Meeran, Hemalatha Srinivasan + Show 4 more

Open Access

https://doi.org/10.1186/s13568-020-01104-5

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Abstract

In this study the aromatic nanocolloids (CANCs) are synthesized using the noble metal silver by using Citronella extract and confirmed through physio chemical analysis. The synthesised CANCs were evaluated for its antimicrobial activity and antibiofilm activity against pathogenic biofilm forming E. coli. In addition, synthesized CANCs were evaluated for the expression of virulent genes encoding AmpC and CTX-M-15. The results confirmed that CANCs showed effective antimicrobial activity through its bacteriostatic, bactericidal and quorum quencher activity and downregulated CTX-M-15 gene. CANCs were validated as alternate to the commercial fungicides to control plant pathogenic fungi such as A. niger MTCC (281), Fusarium graminearum MTCC (2089) and F. udum MTCC (2204). Furthermore, analysis of CANCs on breast cancer (MCF-7) cells under in vitro condition showed that the cytotoxicity of CANCs is dose dependent. Thus, the multifunctional CANCs can be utilized as potential antimicrobial, antifungal and anticancer agent.

Highlights

Nanotechnology is the most important upcoming field in material science and technology
Several physical and chemical methods are used for the stabilization of green nanocolloids, which are nontoxic to plants and animals and toxic to bacterial cell
The strains used for this study are E. coli ATCC (25922), F. graminearum MTCC (2089), F. udum MTCC (2204), A. niger MTCC (281)

Summary

Introduction

Nanotechnology is the most important upcoming field in material science and technology. Several physical and chemical methods are used for the stabilization of green nanocolloids, which are nontoxic to plants and animals and toxic to bacterial cell. Urinary tract infections and intestinal infections are majorly caused by biofilm forming Escherichia coli. Biofilm forming E. coli pathogens will lead to infections such as wounds, pneumonia, implant, catheter-mediated infections which affect people all around the world (Mittal et al 2015). Extracellular polymeric substances (EPS) layer of the Biofilm makes the cell resistant to antibiotics by means of hindering its action upon the organism (Shankar et al 2003; Mittal et al 2015). The immediate and alternate antibiofilm agents are required to combat the disease caused by biofilm forming E. coli. There is a crucial need to eradicate biofilm forming E. coli by means of alternative therapeutic strategies such as nanoparticles (Roy et al 2018)

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