Abstract
A multi-platform detection based on hafnia nanoparticles (HfO2NPs) has been developed for immunosensing of aflatoxin M1 (AFM1) as a model analyte. The fine-grained nanocrystal ceramic powder samples of HfO2NPs were prepared using a solution based chemical method by easily available laboratory reagents. Highly specific monoclonal antibody was immobilized on HfO2 surface using novel chemical modification and crosslinking chemistry. The nano-immunosensor was investigated using the chemiluminescent sandwich enzyme-linked immunosorbent assay in the range 200–0.5 pg mL−1. Simultaneously, the particles were also investigated photometrically. The developed immuno-sensor exhibited good linearity with a limit of detection 6.25 pg mL−1, sensitivity, selectivity and stability. The IC50 value was obtained at 61.48 pg mL−1. Moreover, recovery of milk samples was close to 100%. Using the developed immuno-sensor, AFM1 was measurable even after 30 days at storage condition of 4 °C and as well as room temperature (at 28 °C). The miniaturised nano-immunosensor was used for high-throughput analysis (384 individual samples/40 min) of AFM1. To the best of our knowledge, this is the first report in which HfO2NPs are used for the fabrication of a multi-platform immuno-sensor system for AFM1 in milk.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
