Multi-mycotoxin screening of feed and feed raw materials from Africa

Abstract

As animal feed is prone to infestation with mycotoxin-producing fungi, mycotoxin contamination of feed should be monitored. Here, we report a multi-mycotoxin survey of feed samples from Africa. We determined the concentrations of aflatoxins, fumonisins, deoxynivalenol, T-2 toxin, zearalenone and ochratoxin A in 1,045 samples of finished feed and feed raw materials (maize, maize silage, other cereals, etc.) from South Africa and 318 samples from Algeria, Tunisia, Morocco, Senegal, Côte d’Ivoire, Nigeria, Ghana, Namibia, Uganda, Kenya, Tanzania, Zambia and Madagascar. We compared the measured mycotoxin concentrations to regulatory limits or guidance values that are in effect in the European Union and analysed the co-occurrence of these mycotoxins. To determine the occurrence of other fungal secondary metabolites, a subset of the samples was analysed using a multi-analyte liquid chromatography tandem mass spectrometry-based method for the simultaneous detection of over 700 fungal metabolites. We found that 33.3% of maize samples and 54.4% of finished feed samples from Senegal, Côte d’Ivoire, Nigeria, Ghana, Namibia, Uganda, Kenya and Tanzania exceeded the European regulatory limit of 20 ng/g aflatoxins. TheFusarium mycotoxins zearalenone, fumonisins and deoxynivalenol were prevalent in all commodities from all countries, but concentrations were in most cases below European guidance values. Concentrations of deoxynivalenol and zearalenone were correlated. Several otherFusarium metabolites occurred frequently (e.g. moniliformin, beauvericin, aurofusarin) or in high concentrations (e.g. aurofusarin, fusaproliferin). Furthermore, high levels of diplodiatoxin were occasionally detected in samples from South Africa and theAlternaria metabolite tenuazonic acid was prevalent and reached high concentrations. In conclusion, aflatoxins frequently occurred in African feed samples in potentially unsafe concentrations. WhileFusarium mycotoxins mostly occurred in concentrations below European guidance values, a correlation between deoxynivalenol and zearalenone concentrations suggests that toxicological interactions of these compounds deserve attention. Several less investigated fungal secondary metabolites occurred frequently or reached high concentrations.