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Abstract
We demonstrate multi-mode microscopy based on a single femtosecond fiber laser. Coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and photothermal images can be obtained simultaneously with this simplified setup. Distributions of lipid and hemoglobin in sliced mouse brain samples and blood cells are imaged. The dependency of signal amplitude on the pump power and pump modulation frequency is characterized, which allows to isolate the impact from different contributions.
Highlights
Coherent Raman spectroscopy (CRS) [1] utilizes the signature of intrinsic molecular vibrations to achieve chemical specificity in nonlinear imaging without the need for extrinsic fluorescence labels
We demonstrate multi-mode microscopy based on a single femtosecond fiber laser
The dependency of signal amplitude on the pump power and pump modulation frequency is characterized, which allows to isolate the impact from different contributions
Summary
Coherent Raman spectroscopy (CRS) [1] utilizes the signature of intrinsic molecular vibrations to achieve chemical specificity in nonlinear imaging without the need for extrinsic fluorescence labels. The amplitude of the SRS signal is ISRS ∝ PsPp. the energy transfer is usually very weak, with a relative power change on the order of 10−6 or less, it can be detected by phase sensitive detection techniques such as lock-in amplification [2]. The energy transfer is usually very weak, with a relative power change on the order of 10−6 or less, it can be detected by phase sensitive detection techniques such as lock-in amplification [2] This involves the intensity modulation of the pump (or Stokes) beam at a radio frequency (RF) and measuring the amplitude of the crosstalk induced in the Stokes (or pump) beam at the same frequency of the modulating waveform
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