Abstract
Salmonella enterica subsp. enterica serovar Enteritidis (S. Enteritidis) was responsible for a worldwide pandemic during the 1980s and 1990s; however, changes in the dominant lineage before and after this event remain unknown. This study determined S. Enteritidis lineages before and after this pandemic event in Japan using multilocus sequence typing (MLST). Thirty S. Enteritidis strains were collected in Japan between 1973 and 2004, consisting of 27 human strains from individual episodes, a bovine strain, a liquid egg strain and an eggshell strain. Strains showed nine phage types and 17 pulsed-field profiles with pulsed-field gel electrophoresis. All strains had homologous type 11 sequences without any nucleotide differences in seven housekeeping genes. These MLST results suggest that S. Enteritidis with the diversities revealed by phage typing and pulsed-field profiling has a highly clonal population. Although type 11 S. Enteritidis may exhibit both pleiotropic surface structure and pulsed-field type variation, it is likely to be a stable lineage derived from an ancestor before the 1980s and/or 1990s pandemic in Japan.
Highlights
Other human strains, the liquid egg strain and the eggshell strain were isolated from different samples at the Fukuoka Institute of Health and Environmental Sciences
14b enterica serovar Enteritidis strains in Japan from 1973 to 2004. aST, strain type. bNT, not tested
PTs and genotypes defined by pulsed-field profiles (PFPs), it is likely to be a stable lineage that was derived from an ancestor before the pandemic in Japan
Summary
Enteritidis (defined by phage types, (PTs)) infecting chicken reproductive tissues emerged simultaneously in geographically separate countries during this pandemic. * Correspondence: murakami@fihes.pref.fukuoka.jp 1Division of Pathology and Bacteriology, Fukuoka Institute of Health and Environmental Sciences, 39 Mukaizano, Dazaifu, Fukuoka 818-0135, Japan Full list of author information is available at the end of the article possessed this ability evolved into multiple lineages before the pandemic. Enteritidis before and after the pandemic during the 1980s and/or 1990s in Fukuoka Prefecture, Japan.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
