Abstract
Hydroxyapatite Ca10(PO4)6(OH)2 (HAp) is an important bioactive material for bone tissue reconstruction, due to its highly thermodynamic stability at a physiological pH without bio-resorption. In the present study, the Ag:HAp and the corresponding Ag:HAp + D3 thin films (~200 nm) coating were obtained by vacuum deposition method on Ti substrate. The obtained samples were exposed to different UV irradiation times, in order to investigate the UV light action upon thin films, before considering this method for the thin film’s decontamination. The effects of UV irradiation upon Ag:Hap + D3 are presented for the first time in the literature, marking a turning point for understanding the effect of UV light on composite biomaterial thin films. The UV irradiation induced an increase in the initial stages of surface roughness of Ag:HAp thin film, correlated with the modifications of XPS and FTIR signals. The characteristics of thin films measured by AFM (RMS) analysis corroborated with XPS and FTIR investigation highlighted a process of recovery of the thin film’s properties (e.g., RMS), suggesting a possible adaptation to UV irradiation. This process has been a stage to a more complicated UVA rapid degradation process. The antifungal assays demonstrated that all the investigated samples exhibited antifungal properties. Moreover, the cytotoxicity assays revealed that the HeLa cells morphology did not show any alterations after 24 h of incubation with the Ag:HAp and Ag:HAp + D3 thin films.
Highlights
Hydroxyapatite Ca10(PO4)6(OH)2, and (HAp), is an important compound with remarkable biological properties, mainly used in the biomedical field, as a bioactive material for bone tissue reconstruction, due to its highly thermodynamically stability at a physiological pH (i.e., 7.4), but without bio-resorption [1]
The X-ray photoelectron spectroscopy (XPS) spectra of Ca2p, P2p and Ag3d lines measured from the Ag:HAp/Ti thin films in the initial stage of the sample are as follows, with the observation that the calculated value of the modified Auger parameter using Ag3d and Ag M4N45N45 is 726.4 eV, which clearly indicates the presence of a metallic state
The O1s signal and C1s signal are present in the initial HAp:Ag/Ti films and can be observed in Figure 1e,f, i.e., for O1s (peak at binding energy (BE) of 531.13 eV was assigned to O in HAp; peak at binding energy (BE) of 532.18 eV was associated to C bonds while the peak at BE of 533.30 eV was assigned to OH and H2O traces); for C1s
Summary
Hydroxyapatite Ca10(PO4)6(OH), and (HAp), is an important compound with remarkable biological properties, mainly used in the biomedical field, as a bioactive material for bone tissue reconstruction, due to its highly thermodynamically stability at a physiological pH (i.e., 7.4), but without bio-resorption [1]. The advantage of using HAp as bio implant coating is related to its capability to connect structurally and functionally with the human bone, increasing the osteo-inductivity of medical devices [4]. These biomaterials can be used for the decontamination of polluted water [5,6]. The silver doped HAp (AgHAp) exhibits a dual property, i.e., bacterial inhibition and the enhancement of implant osteo-conductivity [14]
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