Multi-Level Analyses of Genome-Wide Association Study to Reveal Significant Risk Genes and Pathways in Neuromyelitis Optica Spectrum Disorder.

Ting Li,Ming Yi,Qiu-Xia Zhang,He Li,Shu-An Dong,Li Yang,Fu-Dong Shi,Bin Feng,Chun-Sheng Yang,Yue Li

doi:10.3389/fgene.2021.690537

Abstract

BackgroundNeuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system and it is understandable that environmental and genetic factors underlie the etiology of NMOSD. However, the susceptibility genes and associated pathways of NMOSD patients who are AQP4-Ab positive and negative have not been elucidated.MethodsSecondary analysis from a NMOSD Genome-wide association study (GWAS) dataset originally published in 2018 (215 NMOSD cases and 1244 controls) was conducted to identify potential susceptibility genes and associated pathways in AQP4-positive and negative NMOSD patients, respectively (132 AQP4-positive and 83 AQP4-negative).ResultsIn AQP4-positive NMOSD cases, five shared risk genes were obtained at chromosome 6 in AQP4-positive NMOSD cases by using more stringent p-Values in both methods (p < 0.05/16,532), comprising CFB, EHMT2, HLA-DQA1, MSH5, and SLC44A4. Fifty potential susceptibility gene sets were determined and 12 significant KEGG pathways were identified. Sixty-seven biological process pathways, 32 cellular-component pathways, and 29 molecular-function pathways with a p-Value of <0.05 were obtained from the GO annotations of the 128 pathways identified. In the AQP4 negative NMOSD group, no significant genes were obtained by using more stringent p-Values in both methods (p < 0.05/16,485). The 22 potential susceptibility gene sets were determined. There were no shared potential susceptibility genes between the AQP4-positive and negative groups, furthermore, four significant KEGG pathways were also identified. Of the GO annotations of the 165 pathways identified, 99 biological process pathways, 37 cellular-component pathways, and 29 molecular-function pathways with a p-Value of <0.05 were obtained.ConclusionThe potential molecular mechanism underlying NMOSD may be related to proteins encoded by these novel genes in complements, antigen presentation, and immune regulation. The new results may represent an improved comprehension of the genetic and molecular mechanisms underlying NMOSD.

Highlights

Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system that predominantly affects the spinal cord and optic nerves (Wingerchuk et al, 2015)
In aquaporin 4 (AQP4)-positive NMOSD cases, where more stringent p-Values were used in both methods, five shared risk genes were obtained at chromosome 6 (p < 0.05/16,532), comprising Complement factor B (CFB), Euchromatic Histone Lysine Methyltransferase 2 (EHMT2), HLA-DQA1, MSH5, and SLC44A4
The 50 potential susceptibility gene sets of AQP4-positive NMOSD are shown in Table 2 and the top 5 of these genes were same with the ones obtained with more stringent p-Values (p < 0.05/16,532)

Summary

Introduction

Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system that predominantly affects the spinal cord and optic nerves (Wingerchuk et al, 2015). Recent studies on genome-wide association (GWAS) of NMOSD have amplified the understanding of NMOSD. Estrada et al (2018) identified two independent SNP (rs1150757 and rs28383224) in the major histocompatibility complex (MHC) region associated with AQP4-Ab positive NMOSD, the susceptibility genes and associated pathways of NMOSD patients with AQP4-Ab positive and negative have not been elucidated. Neuromyelitis optica spectrum disorder (NMOSD) is an inflammatory disease of the central nervous system and it is understandable that environmental and genetic factors underlie the etiology of NMOSD. The susceptibility genes and associated pathways of NMOSD patients who are AQP4-Ab positive and negative have not been elucidated

Methods

Results

Discussion

Conclusion