Abstract
Egg yolk antibodies (IgY)are analternative to mammalian antibodies, which have been successfully applied in treatment ofdisease, asimmunochemical reagents and asfood additives. A fast, accurate, and easy-to-operate detection method for IgY antibodies is needed. Herein, we developed a rapid and cost-effective colorimetric assay for the ultrasensitive detection of anti-S. aureus IgY antibodies using multi-functional magnetic molecularly imprinted polymers (MMIPs). The prepared MMIPs can recognize, adsorb, and separate the analyte from the matrix efficiently, and oxidize TMB to generate a colorimetric signal within ~ 60min. As low as 0.013mg·mL-1 was able to be detected by using this method. The visual detection limit reached 0.02mg·mL-1. By testing the IgY in milk samples, the feasibility was verified.
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