Abstract
A method for screening illegal adulterated antidiabetics in bitter melon and ginseng soft gels (BMGSG) was developed by multi-fingerprinting profiling analysis. HPLC fingerprints of BMGSG samples were investigated for chemical feature and similarity evaluation. Based on chemical structure and spectroscopic properties, chromatographic behaviors of common antidiabetics were further studied under different HPLC conditions combining with multi-wavelength monitoring with diode array detector (DAD) and fluorescence detector (FLD) to highlight and discriminate illegal adulterants in BMGSG products. Thereby, a hierarchical strategy for screening illegal adulterated antidiabetics was designed by multi-fingerprinting profiling analysis coupled to spectral purity examination. Detection limit of identification (ILOD) and detection limit of spectra (SLOD) were defined in the range of 0.115–2.65 μg/mL and 0.382–2.72 μg/mL, respectively. The method also demonstrated good quantification performance through validation tests. It had been successfully applied to screening real samples, and the results were confirmed by ultra-performance liquid chromatography-quadrupole-orbitrap mass spectrometer (UPLC-Q-Orbitrap/MS). Thus, the proposed method was an efficient and reliable approach for detection of illegal adulterated antidiabetics in the dietary supplement.
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