Abstract
Abstract Rat liver samples were irradiated in air by 4 MeV protons for elemental analysis by X-ray emission spectroscopy. The standard instrumental procedure was modified in our study, resulting in optimum detection limits being attained. The analytical system was accurately calibrated with standard biological reference materials, compressed into 2 mm thick disks. Ten macro and trace elements were quantitatively determined in the tissue specimens : eight essential elements (S, CI, K, Ca, Mn, Fe, Cu, and Zn) and two with undefined biological roles (Br and Kb). Detection limits in the dry tissue samples were estimated to be 0.1 ppm for Mn, Fe, Cu and Zn. The method was employed to examine the effects of a low level of phytate supplementation on the trace element status of the rat liver, since phytate feeding potentiated tumor yields when carcinogens were administered. The results are discussed in terms of current hypotheses.
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