Multi-Drug Resistance Patterns of Wound Causing Bacterial Infections in a Rural Hospital, Sirajganj, Bangladesh

Abstract

Wound infection is considered as one of the most common nosocomial infections in the world. The aim of this study is profiling of bacterial pathogenic isolates from wound infected patients who were admitted to the hospital and commonly to measure antimicrobial susceptibility profiling. An entire of 58 samples were collected for the study. The cultural analysis was done by phenotypic examination, and we performed Gram staining and biochemical examination for identification. The pus and wound swabs were collected aseptically and gold standard microbiological cultural analysis was performed in AlHera General Hospital, Sirajganj, Bangladesh between September 2020 and August 2021. 43(74.1%) of the whole samples yielded positive cultures and only 15(25.9%) samples showed negative growth. Gram-Negative bacteria showed more prevalence than Gram-Positive bacteria which were in number 32 and 11 respectively and in at 74.4% and 25.6%. The most predominant isolate was Escherichia coli 18 (41.9%) and second most was Staphylococcus aureus 12(27.9%), and the rest of other bacterial isolates were Pseudomonas aeruginosa was 5(11.6%), Proteus mirabilis 3(7.0%), Serratia marcescens 2(4.7%), Staphylococcus Saprophyticus 2(4.7%) and Klebsiella pneumoniae 1(2.3%). The isolates were resistant to the commonly used oral antibiotics, namely Azithromycin, Amoxicilin, Ceftriaxone, Ceftazidime, Chloramphenicol, Cefixime, Amoxyclav, Colistin, Cefuroxime, Cloxacillin, Ciprofloxacin, Imipenem, Levofloxacilin, Vancomycin, Linezolid, Meropenem, Erythromycin, Gentamycin, Cephradine, Cotrimoxazole, Moxifloxacin which is very alarming. The pathogens showed remarkable sensitivity against Gentamicin and Meropenem, which at least leaves a window to get treated. This study was performed to hunt out appropriate antibiotic/s for wound infections based on pathogens isolated from wounds of hospital admitted patients in Bangladesh and their antibiotic susceptibility profile against available and frequently prescribed antibiotics.