Multi-drops and Cross-sections, Efficient Methods for Establishing Cell Suspension Culture of Cuminum cyminum L. and Plant Regeneration

Abstract

The current study aims of the current study is to establish cell suspension cultures of the medicinal plant, cumin Cuminum cyminum L. by the application of multiple drops and cross-section techniques. These methods were used in cultivating cell suspensions and in vitro plant regeneration. Leaf, stem, hypocotyl and root explants were cultured in Murashige and Skoog (MS) medium containing different concentrations (0.05, 0.5 and 1.0 mg.l-1) of naphthalene acetic acid (NAA) and (0.05, 0.1, 1.0 and 2.0 mg.l-1) benzyl adenine BA for callus production. The results indicated the high response of cumin, as the percentage of callus initiation was 100%.The plant regeneration percentage reached 91.6%. Moreover, a friable callus of hypocotyls was appropriate for initiation of cell suspension culture in MS medium with 0.5mg.l-1 NAA and 1.0 mg.l-1benzyl adenine (BA). The best density for primordial callus formation was 51.0 ×105 cells ml-1 in both multiple-drops and cross-sections embedding methods. Callus that had been produced from multi drops and sectors had the ability for shoot regeneration. This study clarified the efficiency of these techniques in establishing cell suspension culture and shoot regeneration, which could be promising sources for high production of active compounds in cumin plant.