Abstract
The quantitative analysis of multiple components with a single marker (QAMS) method was firstly established for simultaneous determination of 18 active components in Ilex kudingcha C. J. Tseng by HPLC. Using rutin, isochlorogenic acid A and kudinoside A as internal refererence substances (IRS), compatibility results showed that the relative correction factors (RCFs) of all compounds showed good reproducibility under different chromatographic conditions. On the basis of previous studies, the accuracy of the QAMS method was systematically evaluated by investigating the influences of curve intercept, analytes and IRS concentration. The results showed that the concentration (especially at low level) of analytes and curve intercept were the major influencing parameters for the LRG-QAMS method (LRG = linear regression), whereas the influence of IRS concentration seemed more apparent in terms of the AVG-QAMS method (AVG = average). The two approaches were complementary with each other. In addition, hierarchical clustering analysis (HCA), principal components analysis (PCA) and similarity analysis (SA) were performed to differentiate and classify the samples based on the contents of 18 marker compounds. The results of the different chemometric analyses were completely consistent with each other, and could be supported by the quantification results.
Highlights
IntroductionJ. Tseng (Kudingcha in Chinese), the dried leaves of the genus Ilex in the family
Referring to the chromatograms of HPLC-DAD detection, all chromatographic peaks could be divided into three groups by their corresponding maximum absorption wavelength, that was 210 nm for K1, K2, K3, K4 and K5, 260 nm for R1, R2, R3, R4, R5 and R6, and 326 nm for C1, C2, C3, C4, C5, C6 and C7
Tseng samples from different locations, which were based on the principle of the linear relationship between a detector response and the levels of components within certain concentration ranges. As it shown in the results of evaluation the accuracy of QAMS, we found that the standard method difference (SMD) calculated from LRG-QAMS method for those components, the content of which in Ilex kudingcha C
Summary
J. Tseng (Kudingcha in Chinese), the dried leaves of the genus Ilex in the family. Aquifoliaceae, is widely distributed in the Hainan, Hubei, Guangdong, Guangxi and Hunan provinces of China. It has been used as an ethnomedicine for the treatment of diseases relative to dyslipidosis for millennia [1]. Nowadays it has become a hotspot in traditional Chinese medicine (TCM) research due to its significant effects on cardiovascular-related diseases and metabolic syndrome [2,3].
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