Abstract
Among the many environmental stresses, excessive production of reactive oxygen species (ROS) and the ensuring oxidative stress are known to cause significant cellular damage. This has clinical implications in the onset of type 1 diabetes, which is triggered by the destruction of pancreatic β-cells and is associated with oxidative stress. In this study, we investigated the protective and antioxidative effects of mulberry extract (ME) in insulin-producing pancreatic β-cells. We found that ME protects pancreatic β-cells against hydrogen peroxide (H2O2)-induced oxidative stress and the associated apoptotic cell death. ME treatment significantly reduced the levels of H2O2-induced 2-diphenyl-1-picrylhydrazyl (DPPH) radicals, and lipid peroxidation and intracellular ROS accumulation. In addition, ME inhibited DNA condensation and/or fragmentation induced by H2O2. These results suggest that ME protects pancreatic β-cells against hydrogen peroxide-induced oxidative stress.
Highlights
Diabetes is one of the major metabolic diseases among adults in the developed world
Deficiency of insulin caused by the destruction of pancreatic β-cells induces hyperglycemia, which leads to diabetes and serious pathological effects in humans [25]
We used hydrogen peroxide-induced toxicity of pancreatic β-cells as a model to evaluate the antioxidant activity of mulberry extract (ME), and identified the appropriate concentrations that elicited the greatest protective effects
Summary
Diabetes is one of the major metabolic diseases among adults in the developed world. Type 1 diabetes is classified as insulin-dependent diabetes mellitus, whereas Type 2 is defined as non-insulindependent diabetes mellitus [1]. Recent studies have demonstrated that antioxidants can protect cells by reducing hydrogen peroxide-induced oxidative stress [7], N-acetylcysteine (NAC) is an antioxidant known to protect pancreatic β-cells by scavenging ROS in vitro and in vivo, respectively [8,9]. This indicates the importance of isolating substances with antioxidative activity and characterization of their antioxidant mechanisms. We have investigated the protective effect of mulberry extract (ME) on hydrogen peroxide-induced oxidative damages in pancreatic MIN6N β-cells
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