Abstract
1. Scanning electron microscopy of the intestinal epithelium in the cel showed an important mucus layer over the middle intestine. 2. Ion-selective microelectrodes (Na+, Cl−, K+) demonstrated several standing gradients of ion activities in the mucus layer. 3. The Na+ and Cl− gradients were identical and related to transepithelial NaCl absorption. They were cancelled by ouabain application on the serosal side. 4. Symmetrical substitution of Na+ or Cl− in the luminal and serosal Ringer solution gave results in agreement with the presence of an apical Na+−K+−2Cl− cotransport system, and of K+ channels. 5. The intestinal mucus appeared, at least in the middle part of the intestine, to act as a diffusion barrier essential to K+ recycling.
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