Mucilaginibacter boryungensis sp. nov., isolated from soil

Abstract

A Gram-stain-negative, non-motile, non-spore-forming bacterial strain, BDR-9(T), was isolated from soil collected from Boryung on the west coast of the Korean peninsula, and its taxonomic position was investigated by using a polyphasic study. Strain BDR-9(T) grew optimally at 25 °C, at pH 6.0-7.5 and in the absence of NaCl. Phylogenetic analyses based on 16S rRNA gene sequences revealed that strain BDR-9(T) fell within the clade comprising species of the genus Mucilaginibacter within the phylum Bacteroidetes. 16S rRNA gene sequence similarity values between strain BDR-9(T) and the type strains of species of the genus Mucilaginibacter were in the range 94.0-95.6 %. Strain BDR-9(T) contained MK-7 as the predominant menaquinone and iso-C(15 : 0) and C(16 : 1)ω7c and/or iso-C(15 : 0) 2-OH as the major fatty acids. The DNA G+C content was 44.3 mol%. Differential phenotypic properties and phylogenetic distinctiveness of strain BDR-9(T) demonstrated that this strain is distinguishable from species of the genus Mucilaginibacter. On the basis of phenotypic, chemotaxonomic and phylogenetic data, strain BDR-9(T) is considered to represent a novel species of the genus Mucilaginibacter, for which the name Mucilaginibacter boryungensis sp. nov. is proposed. The type strain is BDR-9(T) ( = KCTC 23157(T) = CCUG 59599(T)).