MTORC1 Controls Phase Separation and the Biophysical Properties of the Cytoplasm by Tuning Crowding

Abstract

Macromolecular crowding has a profound impact on reaction rates and the physical properties of the cell interior, but the mechanisms that regulate crowding are poorly understood. We developed genetically encoded multimeric nanoparticles (GEMs) to dissect these mechanisms. GEMs are homomultimeric scaffolds fused to a fluorescent protein that self-assemble into bright, stable particles of defined size and shape. By combining tracking of GEMs with genetic and pharmacological approaches, we discovered that the mTORC1 pathway can modulate the effective diffusion coefficient of particles ≥20nm in diameter more than 2-fold by tuning ribosome concentration, without any discernable effect on the motion of molecules ≤5nm. This change in ribosome concentration affected phase separation both invitro and invivo. Together, these results establish a role for mTORC1 in controlling both the mesoscale biophysical properties of the cytoplasm and biomolecular condensation.