MTHFR C677T gene polymorphism is not associated with preterm premature rupture of membranes in North Indian women

Abstract

AimThe present study aims to understand the role of methylenetetrahydrofolate reductase (MTHFR C677T) gene polymorphism in preterm premature rupture of membranes. MethodsThe preterm premature rupture of membranes (N = 90) cases and their gestation matched controls (N = 122) were recruited from Lady Hardinge Medical College and Smt. Sucheta Kriplani Hospital. Fasting blood sample (~5 ml) was drawn and genotypic analysis of MTHFR C677T polymorphism was performed by PCR-RFLP in all the PPROM cases and controls. Fetal genotypic analysis of MTHFR C677T was performed using placental DNA in PPROM cases (N = 16) and controls (N = 23). ResultsThe genotypic frequency distribution of MTHFR C677T gene polymorphism of normal homozygous CC genotype was found to be similar among cases (76.67%) and controls (72.27%). Further, the analysis revealed a lower percentage of mutant heterozygotes (CT = 23.33%) among cases as compared to controls (CT = 27.73%). The cases and controls were not found to carry homozygous mutant TT genotype. ConclusionMTHFR C677T gene polymorphism does not seem to play a major role in association with preterm premature rupture of membranes in the North Indian women.