MTA-Enriched Polymeric Scaffolds Enhanced the Expression of Angiogenic Markers in Human Dental Pulp Stem Cells.

Mahdieh Alipour,Zahra Aghazadeh,Mehdi Hassanpour,Marjan Ghorbani,Roya Salehi,Marziyeh Aghazadeh,Yuriy Petrenko

doi:10.1155/2022/7583489

Mahdieh Alipour, Zahra Aghazadeh + Show 5 more

Open Access

https://doi.org/10.1155/2022/7583489

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Abstract

Revascularization of the pulp tissue is one of the fundamental processes and challenges in regenerative endodontic procedures (REPs). In this regard, the current study is aimed at synthesizing the mineral trioxide aggregate- (MTA-) based scaffolds as a biomaterial for REPs. Poly (ε-caprolactone) (PCL)/chitosan (CS)/MTA scaffolds were constructed and evaluated by FTIR, SEM, XRD, and TGA analyses. Proliferation and adhesion of human dental pulp stem cells (hDPSCs) were assessed on these scaffolds by scanning electron microscopy (SEM) and MTT assays, respectively. The expression of angiogenic markers was investigated in gene and protein levels by real-time PCR and western blotting tests. Our results indicated that the obtained appropriate physicochemical characteristics of scaffolds could be suitable for REPs. The adhesion and proliferation level of hDPSCs were significantly increased after seeding on PCL/CS/MTA scaffolds. The expression levels of VEGFR-2, Tie2, and Angiopoietin-1 genes were statistically increased on the PCL/CS/MTA scaffold. In support of these findings, western blotting results showed the upregulation of these markers at protein levels in PCL/CS/MTA scaffold (P < 0.05). The current study results suggested that PCL/CS/MTA scaffolds provide appropriate structures for the adhesion and proliferation of hDPSCs besides induction of the angiogenesis process in these cells.

Highlights

The treatment of necrotic and inflamed immature permanent teeth has been considered a challenging clinical situation [1]
Phosphate-buffered saline (PBS), fetal bovine serum (FBS), trypsin-EDTA solution, high-glucose Dulbecco’s modified Eagle’s medium (DMEM/HG), and penicillin/streptomycin were obtained from Gibco. 3-(4,5-Dimethylthiazol-2yl-2,5-diphenyltetrazolium bromide) (MTT) powder was obtained from Invitrogen (Carlsbad, CA, USA)
The Fourier transform infrared spectroscopy (FTIR) spectrum of MTA presented the specific bands of PO43- from stretching and bending mode (673 cm-1, 918 cm-1) and OH

Summary

Introduction

The treatment of necrotic and inflamed immature permanent teeth has been considered a challenging clinical situation [1]. The limitations of this method are the difficulty of applying apical stops, preventing root development, and increasing the risk of tooth fracture [2, 3]. To overcome these limitations, regenerative endodontic procedures (REPs) have been introduced as a promising approach for the regeneration of the dentin-pulp complex [4,5,6]. Pulp tissue is a highly vascularized tissue surrounded by dentin Due to this architecture of the dentin-pulp complex, the angiogenesis procedure during tissue regeneration is challenging [9]

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Conclusion