Abstract
Calvin Tiengwe works on posttranscriptional gene regulation and iron homeostasis in the parasitic protozoan Trypanosoma brucei In this mSphere of Influence article, he reflects on how the paper "Comprehensive identification of RNA-protein interactions in any organism using orthogonal organic phase separation (OOPS)" by Queiroz et al. (Nat Biotechnol 37:169-178, 2019, https://doi.org/10.1038/s41587-018-0001-2) influenced his research by providing a tool to capture RNA-protein complexes on a global scale using acid guanidinium thiocyanate-phenol-chloroform (AGPC), an old method hitherto applied for RNA, DNA, or protein purification.
Highlights
Starting an academic research career as an independent investigator comes with great enthusiasm and momentum to begin performing experiments
Queiroz et al reasoned that prior to lysis with acid guanidinium thiocyanate-phenol-chloroform (AGPC), irradiating cells with UV at 254 nm will result in accumulation of RNA-protein complexes at the interface
The new method is efficient, reproducible, and less expensive, and its broad application in any organism is attractive for my research in that it could be adopted to identify novel posttranscriptional regulatory factors of iron deficiency in T. brucei
Summary
Starting an academic research career as an independent investigator comes with great enthusiasm and momentum to begin performing experiments. An mSphere of Influence commentary by @Tiengwe_Lab on a new method for capturing RNA-protein complexes on a global scale. Queiroz et al reasoned that prior to lysis with AGPC, irradiating cells with UV at 254 nm will result in accumulation of RNA-protein complexes at the interface.
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