Abstract
Objective: To analyze the methylation status of the genes MSH2, MSH3, and MSH6 in ameloblastomas. Study Design: Fresh samples of ameloblastoma (group 1, n = 10) and dental follicles (group 2, n = 10) were digested and their DNA was isolated for quantitative polymerase chain reaction (qPCR). Methylation assessment was performed by EpiTect Methyl II PCR Assays (Qiagen). We also performed immunohistochemical reactions for MSH2, MSH3, and MSH6 in paraffin sections (group 1). The results were digitally quantified in all ameloblastomas. Results: The mean methylation profile of the genes MSH2, MSH3, and MSH6 in ameloblastoma was not statistically different from dental follicles. However, a negative correlation for MSH2 (r = –0.7455; P = .0174) and MSH6 (r = –0.8552; P = .0016) protein expression with the methylation profile was found in ameloblastoma. Conclusion: Although the methylation profile of the genes in ameloblastoma was not different from dental follicles, our study shows that epigenetic events are potential regulators of MSH2 and MSH6 protein expression in ameloblastomas. This study was supported by FAPESP.
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